Seminario del Departamento de Microbiología
Paper review: Chronic Exposure to Petroleum-Derived Hydrocarbons Alters Human Skin Microbiome and Metabolome Profiles: A Pilot Study………..Daniel A. Murillo………..Skin exposure to petroleum hydrocarbons (PH) is linked both to significant alteration of skin microbiota and strong association with skin diseases like dermatitis and melanoma. This interaction is suggested to be a pathway to proliferation of oil-degrading bacteria and subsequent synthesis of cyto- and genotoxic metabolites: higher exposure, higher microbiome alterations, higher production of toxic metabolites. Hernández-Melgar et. al. (2024) aimed to characterize bacterial communities and its metabolome in skin areas exposed to PH, with auto repair and tire shop workers as case group and non-exposed individuals as control group.
Samples were collected using cotton swabs for DNA extraction and split in two sets: the first for LC-MS2 based untargeted metabolomics and chemoinformatics; the latter for 16S rRNA sequencing, in order to obtain a panorama of bacterial and metabolic diversity in exposed hands (EH) and non-exposed hands (NEH), with a subcohort in arms as an intrasubject control. After characterization of bacterial communities and metabolites, data analysis was conducted to evaluate relations between bacterial genera and metabolites in different skin areas.
23 bacterial phyla were identified. Alpha diversity analysis demonstrated a higher diversity in exposed anatomical sites compared to non-exposed areas. Proteobacteria represented the largest phyla, with 64.80% of relative abundance. 397 bacterial genera were identified. Eight of them thrived in EH. Dietzia, Kocuria and Paracoccus were the most abundant. The bacterial profile was associated with 45 KEGG upregulated modules in EH, most of them associated with hydrocarbon metabolism. In comparison, only 7 were altered in NEH and non-exposed arms (NEA). 861 metabolomic features were obtained using MZmine software. The most notorious dysregulated chemicals were lipid metabolites. While EH was associated with a predominant skin metabolome composition of phenol ethers, NEH presented abundance of glycerolipids.
PH exposure was correlated with ODB proliferation and a metabolomical shift from lipidical metabolites to molecules capable of potential skin barrier dysregulation………10.1021/acs.jproteome.4c00256………….https://cicese.zoom.us/j/87006389972?pwd=9sWluAL27rbgpZEqYcJFkuYZgo8Qcr.1
Lugar: Auditorio Ildiko Bartnicki
Fecha: 04-02-2026
Hora: 09:00 am
