Seminario de Microbiología

In situ architecture of the ER-mitochondria encounter structure……………………………………Juan Manuel Martinez Andrade……………………The findings of this research paper revealed the in situ supramolecular architecture of a major interorganelle lipid transfer machinery and provide a basis for the mechanistic understanding of lipid fluxes in eukaryotic cells. In yeast, tethering and lipid transfer between the two organelles is mediated by the endoplasmic reticulum-mitochondria encounter structure (ERMES), a four-subunit complex of unresolved stoichiometry and architecture. In this article, the authors determined the molecular organization of ERMES within Saccharomyces cerevisiae cells using integrative structural biology by combining quantitative live imaging, cryo-correlative microscopy, subtomogram averaging and molecular modelling. For first time, it was revealed how ERMES assembles into approximately 25 discrete bridge-like complexes distributed irregularly across a contact site. Moreover, the article described how each bridge consists of three synaptotagmin-like mitochondrial lipid binding protein domains oriented in a zig-zag arrangement. Finally, the authors published a molecular model of ERMES that reveal a pathway for lipids………………………………https://doi.org/10.1038/s41586-023-06050-3................................................ https://zoom.us/j/6983908886?pwd=xRlDLa9JKuEOttMI0PCJ4uJDjizFXg.1

Lugar: Sala de Usos Múltiples-SUM

Fecha: 20-11-2024

Hora: 09:00 am